CENTER FOR ELECRON MICROSCOPY

Center for Electron Microscopy is an infrastructural center dedicated for research and higher education in electron microscopy. Electron microscopy is used for structural and functional analyses of medical and biological samples. The main aims of the Center are (1) providing high-quality service and technical expertise in transmission and scanning electron microscopy, (2) conducting basic and practical courses and training on electron microscopy, and (3) assisting in cross-disciplinary research on the development and applications of advanced methodologies in electron microscopy, (4) light microscopy of live and fixed cells and tissue (brightfield and confocal microscopy).

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Our electron microscopy technologies we offer:

• Chemical and metal-mirror cryofixation

• Embedding in epoxy resin

• Freeze-substitution, progressive lowering of temperature and low temperature embedding

• Ultramicrotomy and cryo-ultramicrotomy

• Preembedding and postembedding immunolabelling

• Negative staining

• Freeze-fracturing, freeze-etching and freeze-fracture replica immunolabelling

• Critical point drying

• Correlative light and electron microscopy

 

Our light microscopy technologies we offer:

• Preparation of tissue for light microscopy (paraffin sections, cryostat sections)

• Immunocytochemistry / immunofluorescence labelling

• Live cell imaging

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Electron microscopy instruments:

• Scanning electron microscope SEM Tescan Vega 3

• FEI Quanta 250 FEG Scanning Electron Microscope

• Transmission electron microscope Philips CM100 with ATM CCD camera

• MM80 metal-mirror cryofixation device

• Leica EM ICE high pressure freezing system

• Leica EM AFS freeze-substitution and embedding system

• Leica EM AFS2 with Leica EM FSP (Automatic Freeze Substitution Processor) with integrated UV LEDs

• Critical point dryer

• Freeze-fracturing device (Balzers)

• Leica Ultracut UCT microtome

• Leica EM UC6 ultramicrotome

• Leica UC7 ultramicrotome/FC7 cryo-ultramicrotome

 

Light microscopy instruments:

• Leica microsystems DM IL (inverted microscope)

• Nikon  (inverted microscope)

• Inverted confocal microscope LSM 900 Airyscan, Carl Zeiss with the metabolic chamber for live cell imaging

• Widefield fluorescence microscope Axio Imager Z1 Carl Zeiss with Axio Vision software, and structured illumination (ApoTome)